Publication details.

Paper

Year:2018
Author(s):M. Mulet, D. Sánchez, A. Rodríguez, B. Nogales, R. Bosch, A. Busquets, M. Gomila, J. Lalucat, E. García-Valdés
Title:Pseudomonas gallaeciensis sp. nov., isolated from crude-oil-contaminated intertidal sand samples after the Prestige oil spill
Journal:SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN:0723-2020
JCR Impact Factor:2.808
Volume:41
Issue No.:4
Pages:340-347
D.O.I.:10.1016/j.syapm.2018.03.008
Web:https://dx.doi.org/10.1016/j.syapm.2018.03.008
Abstract:© 2018 Elsevier GmbH Strains V113T, V92 and V120 have been isolated from sand samples taken at the Atlantic intertidal shore in Galicia, Spain, after the Prestige oil spill. A preliminary analysis of the 16S rRNA and the partial rpoD gene sequences indicated that these strains belonged to the Pseudomonas genus, but they were distinct from any known Pseudomonas species. They were extensively characterized by a polyphasic taxonomic approach and phylogenetic data that confirmed that these strains belonged to the Pseudomonas pertucinogena group. Phylogenetic analysis of 16S rRNA, gyrB and rpoD gene sequences showed that the three strains were 99% similar and were closely related to members of the P. pertucinogena group, with less than 94% similarity to strains of established species; Pseudomonas pachastrellae was the closest relative. The Average Nucleotide Index based on blast values was 89.0% between V113T and the P. pachastrellae type strain, below the accepted species level (95%). The predominant cellular fatty acid contents and whole cell protein profiles determined by MALDI-TOF mass spectrometry also differentiated the studied strains from known Pseudomonas species. We therefore conclude that strains V113T, V92 and V120 represent a novel species of Pseudomonas, for which the name Pseudomonas gallaeciensis is proposed; the type strain is V113T (= CCUG 67583T = LMG 29038T).

Related staff

  • Balbina Nogales Fernández
  • Jorge Lalucat Jo
  • Rafael Bosch Zaragoza
  • Elena García-Valdés Pukkits
  • Related departments

  • Animal and Microbial Biodiversity
  • Related research groups

  • Environmental Microbiology