Marine mammals and turtles are often studied using the carcasses of stranded dead
individuals. Since decomposition processes might modify the stable isotope ratios of tissues, the
present study tested the effects of decomposition on carbon (δ13C) and nitrogen (δ15N) stable isotope
ratios in 2 tissue types of striped dolphin Stenella coeruleoalba and loggerhead sea turtle
Caretta caretta. Decomposing carcasses of 3 dolphins and 3 turtles were sampled for muscle and
skin for 62 d. Following lipid extraction, samples were analysed regularly using a continuous flowisotope
ratio mass spectrometer. Samples reached decomposition stage CC4 after 62 d at ambient
temperature, but no statistical change in δ13C or δ15N was observed over that period for either tissue
or species. These results imply that muscle and skin samples from carcasses decomposing out
of water at stage CC4 or lower can be used as reliable material for stable isotope analysis in these
2 species, and probably in other marine mammal and marine turtle species. The effects of decomposition
on the stability of stable isotope ratios in other tissues, in carcasses at stage CC5, or in carcasses
decomposing underwater require further study.
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